WebJan 1, 2011 · Cell culture media: SAGM small airway epithelial cell growth media (SABM) supplemented with SAGM SingleQuots for SAEC, and BEGM bronchial epithelial cell growth media (BEBM) supplemented with BEGM SingleQuots (both Lonza). ... 50% SABM/BEBM (Lonza) and 50% DMEM high glucose (Gibco BRL). 2.3 Reagents for Barrier Disruption. 1. WebLonza is a leading global provider of Bioscience solutions. We focus on enabling treatments that prevent illness and support healthier lifestyles. We optimize scientific …
Lonza™ Clonetics™ Bronchial Epithelial Cell Growth …
WebDec 9, 2016 · Beas-2B cells were cultured in BEBM media (Lonza Inc., Allandale, NJ, USA) supplemented with a BEGM kit (Lonza Inc.) without the gentamycin-amphotericin B mix. Cigarette smoke extract (CSE) was made essentially by the method detailed in Eurlings et al. . Filters were removed from 3R4F Research cigarettes (University of Kentucky, … WebThe base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit. ... While other unspecified media and reagents may also produce satisfactory results, a change ... burns practice goodison boulevard doncaster
BEBM Bronchial Epithelial Basal Medium from Lonza
WebBEBM media (Lonza, Walkersville, MD) supplemented with BEGM SingleQuotsTM Supplements and Growth Factors (Lonza). Cells were maintained at 37°C, 5% CO2 … WebLonza Walkersville, Inc. Walkersville, MD 21793-0127 USA U.S. Scientific Support: 800 521 0390 [email protected] EU/ROW Scientific Support: +32 87 321 611 … Webin complete BEGM (Lonza) with growth factor supplements in sub-merged monolayer culture and then seeded at 2 510 cells in trans-wells (cat. no. 3460, Corning) in a 12-well plate with ALI-initial media comprised of 50% BEBM-50% DMEM containing 0.1% hydrocorti-sone, 0.1% bovine insulin, 0.1% epinephrine, 0.1% transferrin, 0.4% burn spray