Web(Beckman, A63882) was used to clean the rst 15 cycles amplicons and puried PCR products were then amplified for a second round (25 cycles) with a pair of communal primers. Libraries were loaded onto a BGIseq500 (BGI-Shenzhen, Shenzhen, China) and underwent 200 bp single end to perform sequencing. A mean of 5,971,100 (range WebDNA library was constructed through end-repair, dA-tailing, adaptor-ligation and 13 cycles PCR amplification followed a final purification by AMPure XP beads (Beckman, A63882). Subsequently, the resulting libraries were denatured and neutralized and subjected to 116 bp single-end sequencing on an Illumina NovaSeq platform (Illumina, USA) by Chengdu …

Effective ribosomal RNA depletion for single-cell total RNA-seq ... - PeerJ

WebJul 29, 2024 · Sheared DNA was cleaned up with 1.5× Agencourt AMPure XP beads (A63882, Beckman Coulter). Gene Read DNA Library I Core Kit (180434, Qiagen) was used for end repair, A-addition and adapter ligation. WebBuy Beckman Coulter A63881 REAGENT, 60 ML CONTAINER, LIQUID at PartsSource. Largest Online marketplace for Medical Equipment Replacement Parts featuring OEM … gluten free red wines

PCR Reaction Setup and AMPure XP Application

WebBeckman美国贝克曼库尔特有限公司是全球专业仪器生产，销售和服务厂家，公司创立于1935年，在过去的七十多年中，公司以不断向生物医学研究，药物开发和临床诊断领域的科学家和医务工作人员提供简单化，自动化和创新的解决方案为己任，从一个小小的酸度计的生产开始，发展到一个拥有数十种 ... WebThe Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. High recovery of amplicons, greater than 100 bp. Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants. No PCR degradation after storage at 4°C for ... WebFlow-Set Pro Fluorospheres is a suspension of fluorescent microspheres used as an aid in standardizing the Navios™ or FC 500 Flow Cytometer. Request a Quote. bold plumbing columbus texas