Biotopped sds
WebApr 5, 2024 · The cells were incubated with hybridization buffer, which contain 50% formamide (Biotopped), EDTA-2 × SSC (Sigma-Aldrich), 300 mM sodium chloride (pH 7.0), and 10% dextran sulfate (Biotopped), at 66 °C for an hour. ... The membrane was washed with 2 × SSC and 0.1% SDS twice at 65 °C for 20 min each, and then washed with 1 × … WebFeb 9, 2024 · biotope: [noun] a region uniform in environmental conditions and in its populations of animals and plants for which it is the habitat.
Biotopped sds
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WebMar 25, 2024 · Protein bands were separated by SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, MA, United States). Sealed in Tris-buffered saline (TBS) containing 5% bovine serum albumin (BSA) (Biotopped, Beijing, China) at room temperature for 1 h, then incubated with primary antibodies at 4°C … WebNov 18, 2024 · In contrast, SDS-PAGE gel shows a single band at ~10 kDa for both peaks, ... (Biotopped Life Sciences), was used to measure the free sulfhydryl groups in the protein solution. DTNB was dissolved ...
Webdialysis bag method at 37 C. The dialysis bag (Biotopped, USA) with a molecular weight cut-off size of 8000–14000 Da was used, and PBS (pH 7.4) containing 0.2% (w/v) sodium dodecyl sulfate (SDS) was employed as dissolution medium. The dialysis bag could retain SLNs while allowing the diffusion of free drug into the dissolution medium. In brief, WebJan 20, 2024 · SDS–PAGE was performed on an electrophoresis apparatus (Bio-Rad Laboratories, Hercules, CA, USA), with a 12% (w / v) acrylamide separating gel and a 5% (w / v) stacking Quick SDS-PAGE Gel Preparation Kit, according Laemmli’s method . SDS-PAGE was performed by electrophoresis, at a constant voltage of 100 V, when the …
WebJan 26, 2024 · Purified rCap protein and pET-32a vector protein as controls were separated on an SDS–polyacrylamide gel, and the samples were transferred to pure nitrocellulose blotting membranes (Pall Corporation, USA) for Western blot. ... After the membranes were washed again with PBS–T, the bands were detected using DAB reagents (Biotopped, … WebNov 2, 2024 · Aconitine linoleate (1) is a lipo-diterpenoid alkaloid, isolated from Aconitum sinchiangense W. T. Wang. The study aimed at investigating the anti-proliferative efficacy and the underlying mechanisms of 1 against MCF-7 and MCF-7/ADR cells, as well as obvious the safety evaluation in vivo. The cytotoxic activities of 1 were measured in vitro. …
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