Flag tag mouse monoclonal antibody
WebThe DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this … WebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ...
Flag tag mouse monoclonal antibody
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WebThis antibody can also be used in the immunohistochemical analysis of mouse tissues containing FLAG peptide without any background staining (PMID: 28377223). 2H8 was … WebThe antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this …
WebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … WebDescription: Antibody for the detection of FLAG™ conjugated proteins (MOUSE) Monoclonal Antibody - 200-301-B13 Synonyms: mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys Tested Applications: ELISA, WB Suggested Applications: FC, IF, IP - View References …
WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. WebFlag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the …
WebMany recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This antibody can detect either N-term or C-term HA tag. 产品编号. 产品名称. 包装. AG8057. HA Tag Rabbit Monoclonal Antibody. 50µl. AZ050.
WebMouse monoclonal [F-tag-01] antibody to DDDDK Tag. Applications: ICC, WB: Reactivity: Species independent: Conjugate: Unconjugated: ... Anti-FLAG Tag Antibody [FG4R] (A85282) 10µg - 200µg / £90 – £280 1 Citation. Description: Mouse monoclonal (FG4R) antibody to DYKDDDDK Tag. Applications: harry reynolds ghanaWebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived … charles poths jrWeb4C5 Anti-DDK Antibody - OriGene’s anti-tag antibodies are developed with high standards of production procedure and validation. For example, 4C5 anti-DDK antibody is generated to detect the DYKDDDDK epitope (same epitope as Flag®). This antibody has been verified for Western blot, immunoprecipitation, immunofluorescence and flow cytometry. harry reynolds floridaWebOur DYKDDDDK Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Rat, Goat and Chicken. These … charles postles jr. delaware representativeWebAntibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope. ELISA. Use a concentration of 1 - 5 µg/ml. As coating antibody in sELISA. charles p. otstottWebAll lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) at 1/1000 dilution Lane 1 : HEK293 cell lysate non-transfected Lane 2 : HEK293 cell … harry reynolds toowoombaWebFlag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the … harry rheam obituary