Mitotracker flow cytometry
WebAfter treatment, cells were incubated with 500 nM MitoTracker Orange CM-H2TMRos for 30 min and fluorescence in the PE channel was measured by flow cytometry. The results are presented as the mean ± SD ( n = 4). *** p ≤ 0.001 as compared with the untreated non-stimulated cells calculated by one-way ANOVA, Dunnett’s test. WebFlow Cytometry Conjugate Alexa Fluor™ 488, MitoTracker™ Red For Use With (Equipment) Fluorescence Microscope, Flow Cytometer Contents & Storage Contains 1 vial of annexin, Alexa Fluor™ 488 conjugate (250 …
Mitotracker flow cytometry
Did you know?
Web9 mrt. 2015 · ing it difficult to quantitate the number of lysosomes or their pH by flow cytometry or fluorometry. Table 2 Summary of our LysoTracker® and LysoSensor™ probes. Cat. no. Probe Abs * (nm) Em * (nm) pK a L7525 LysoTracker® Blue DND-22 373 422 NA L12490 LysoTracker® Blue-White DPX 380 † NA L7526 LysoTracker® Green … Web29 nov. 2024 · Localization, titration and toxicity of MitoTracker Deep Red staining in A549 cells. a Localization of MitoTracker Deep Red staining (red) in parental (top) and paraclonal A549 cells (bottom) analyzed by immunofluorescence microscopy. Cells were counterstained with DAPI. b Titration of MitoTracker Deep Red staining. Flow …
Web13 apr. 2024 · Commercial LysoTracker (LysoTracker Green), MitoTracker, and lipid droplet trackers BODIPY were incubated with CBT-SiPc, ... Flow cytometry analysis of CBT-SiPc-mediated, PDT-induced MCF-7 cells apoptosis/necrosis mechanism before and after irradiation (671 nm, 100 mW/cm 2, 10 min). Web11 jun. 2024 · We therefore conclude that flow cytometry is a suitable method to analyse mitochondrial properties after viral infection. Figure 1 Viral infection changes mitochondrial size and membrane...
WebMitochondria perform crucial roles in many biochemical processes, and mitochondrial depolarization is an early sign of platelet apoptosis. The mitochondrial membrane … Web21 dec. 2024 · Mitotracker Green FM is a mitochondria-specific probe and indicator of mitochondrial mass, targeting these organelles independent of mtROS levels and mitochondrial membrane potential. For measurements of mitochondrial membrane potential TMRE staining was used with or without treatment with FCCP, which depolarizes …
WebMitoProbe Transition Pore Assay Kit for flow cytometry. The flow cytometry histograms show the actions of the various kit components. Jurkat cells were incubated with the …
WebMitoTracker green (MTG) and nonylacridine orange (NAO) are fluorescent probes which have been commonly used to assess mitochondrial mass. This is based on the assumption that both MTG and NAO accumulate in mitochondria regardless of the mitochondrial transmembrane potential (ΔΨm). bricklayer\u0027s ouWebNational Center for Biotechnology Information covid booster vaccine/appointment albertsonsWebFlow cytometry analysis of mitophagy in cell lines. CCCP is the most commonly used inducer of mitophagy in mammalian cells and is thought to exert its effects by reducing the mitochondrial membrane potential. 10 We used low doses of CCCP to induce mitophagy in SH-SY5Y neuroblastoma cells, a Park2 -expressing cell line. covid booster vaccine/appointment at krogerWebThe mitochondrial membrane potential(MMP)was detected by flow cytometry analysis . The ultrastructure of mitochondria and mitophagy vacuoles was observed by transmission electron microscopy(TEM). Auto-phagic vacuoles were observed by dansylpentanediamine(MDC)staining and the expression of autoph-agy related protein … covid booster timing after covid infectionWeb30 mrt. 2016 · MitoTracker has emerged as a useful tool in evaluating mitophagy. Mitochondrial potential-independent MitoTracker is able to stain live mitochondria and enables demonstration of colocalization of … covid booster under age 5Web18 mei 2011 · MitoTracker Green FM reacts with the free thiol groups of cystein residues belonging to mitochondrial proteins ( 18 ). The concentration of this dye is ∼300 times higher in the mitochondrial matrix than in the medium surrounding these organelles, which may be due to the pH of the inner mitochondrial membrane. covid booster vaccination nederlandWeb1 sep. 2015 · Detection of Mitochondrial Mass, Damage, and Reactive Oxygen Species by Flow Cytometry The reagents and procedures highlighted here will give the … covid booster vaccinatie nl