WebAug 1, 2024 · The aim of this review is to evaluate the progress of the phosphorescent Ir(III) complexes in bioimaging applications. We intend to highlight the use of Ir(III) complexes for staining the subcellular organelles, providing phosphorescence lifetime imaging microscopy, two photon and hypoxic tumor bioimaging. WebOrganic room-temperature phosphorescence (RTP) materials are currently the focus of research in the field of bioimaging. In comparison with the conventional imaging …
FLIMJ: An open-source ImageJ toolkit for fluorescence lifetime …
WebNov 19, 2024 · For the first time, the time-resolved two-photon excited autophosphorescence of non-labeled biological specimens was investigated by phosphoresce lifetime imaging with microscopic spatial resolution. A modified multiphoton tomograph was employed to record both photoluminescence contributions, … WebSep 4, 2024 · The reversible tracking of introduced exogenous labile Zn 2+ in MCF-7 and HeLa cells via phosphorescence imaging and phosphorescence lifetime imaging (PLIM) have been realized with Zin-IrDPA. Moreover, PLIM with Zin-IrDPA is able to track the SNOC-stimulated endogenous Zn 2+ release in mitochondria. MeSH terms Binding Sites HeLa … smallest dog in the world ever recorded
Dual-Emissive Phosphorescent Polymer Probe for Accurate …
WebDec 24, 2024 · Lifetime imaging methods using phosphorescence quenching by oxygen for molecular oxygen concentration measurement have been developed and used for noninvasive oxygen monitoring. Webphosphorescence lifetimes can range from hundreds of ns to ms. From a practical point of view, measure - ments of all lifetime transitions involving an emissive process with a lifetime >100 ns have similar require-ments and are therefore covered here, even if the process in question may not be phosphorescence in a strict quantum mechanical sense. A Nikon Ti-E microscope equipped with a Becker & Hickl 405 nm 40 ps diode laser (variable repetition rate 1–80 MHz). PLIM Images were acquired with a 60X (NA 1.20) water immersion objective. PLIM data were acquired using a Becker & Hickl DCS120 confocal unit and a TCSPC SPC150 (Becker & Hickl GmBH) … See more All reagents, unless otherwise stated, were purchased from Thermofisher Scientific. A stock solution of GFP (a kind gift from Prof Cameron Nolan, Science and … See more Recombinant DNAs (cDNA) of Ku70 and Ku80 were bought from Origene, Maryland, USA. The linearized vectors, pOPINE (from Oxford Protein Production Facility, … See more All cell culture reagents, unless otherwise stated, were purchased from Thermofisher Scientific. Cells were grown under humidified 5% CO2in air at 37 °C and … See more Ku80-GFP stably expressing V15B cells (a gift from Dr. Dick van Gent) were passaged grown in phenol red free minimal essential medium (MEM) supplemented with … See more smallest dog in history